rabbit anti ift88 (Proteintech)

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Proteintech rabbit anti ift88
Rabbit Anti Ift88, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+ift88/IFT88+Antibody/pm41677660-116-5-9
Average 96 stars, based on 449 article reviews

rabbit anti ift88 - by Bioz Stars, 2026-09

96/100 stars

Images

Immunofluorescence:

Article Title: Kinase activity of DYRK family members is required for regulating primary cilium length, stability and morphology.
Article Snippet: .. Other antibodies used for immunofluorescence in this study were mouse anti-tubulin (GTU-88; Sigma-Aldrich-T6557) at 1:2000, mouse antiacetylated tubulin (Santa Cruz Biotechnology, sc-23950) at 1:10,000, mouse anti-FLAG IgG1 (Proteintech, 66008-3-Ig) at 1:1000, rabbit antiArl13b (Proteintech, 17711-1-AP) at 1:500, mouse anti-glutamylated tubulin (Adipogen, AG-20B-0020 clone GT335) at 1:1000 and rabbit anti-IFT88 (Proteintech, 12780-1-AP) 1:1000. .. Following primary antibody incubation and 3× PBS wash, coverslips were incubated with Alexa Flour 488-, 568-, or 633-coupled (Life Technologies), and they were used at 1:2000.

Blocking Assay:

Article Title: CEP76 impairment at the centrosome-cilium interface contributes to a spectrum of ciliopathies.
Article Snippet: .. Primary antibodies were diluted in blocking buffer and used in different combinations: Rabbit anti- CEP76 (1:400, Bethyl, A203- 306A), rabbit anti- ARL13B (1:300, Proteintech, 17711- 1- AP), mouse anti- ARL13B (1:300, Proteintech, 66739- 1- Ig), mouse anti–γ- tubulin (1:10,000, Sigma- Aldrich, T6557), rabbit anti- IFT88 (1:500, Proteintech, 13967- 1- AP), rabbit anti- CCP110 (1:200, Proteintech, 12780- 1- AP), and rabbit anti- CEP290 [1:500 (2 μg/ml); Bethyl, A301- 659A]. .. Cells were washed 3× with PBS, blocked with blocking buffer, then incubated with goat anti- rabbit Alexa Fluor 488 (1:500, Thermo Fisher Scientific)– or goat anti- mouse Alexa Fluor 555 (1:500, Thermo Fisher Scientific)–labeled secondary antibodies (1:500, Invitrogen), and counterstained with Hoechst 34580 (1:5000, Thermo Fisher Scientific).

Article Title: CEP76 impairment at the centrosome-cilium interface contributes to a spectrum of ciliopathies
Article Snippet: .. Primary antibodies were diluted in blocking buffer and used in different combinations: Rabbit anti-CEP76 (1:400, Bethyl, A203-306A), rabbit anti-ARL13B (1:300, Proteintech, 17711-1-AP), mouse anti-ARL13B (1:300, Proteintech, 66739-1-Ig), mouse anti–γ-tubulin (1:10,000, Sigma-Aldrich, T6557), rabbit anti-IFT88 (1:500, Proteintech, 13967-1-AP), rabbit anti-CCP110 (1:200, Proteintech, 12780-1-AP), and rabbit anti-CEP290 [1:500 (2 μg/ml); Bethyl, A301-659A]. .. Cells were washed 3× with PBS, blocked with blocking buffer, then incubated with goat anti-rabbit Alexa Fluor 488 (1:500, Thermo Fisher Scientific)– or goat anti-mouse Alexa Fluor 555 (1:500, Thermo Fisher Scientific)–labeled secondary antibodies (1:500, Invitrogen), and counterstained with Hoechst 34580 (1:5000, Thermo Fisher Scientific).

Article Title: Trisomy 21 alters ciliary localization of Sonic Hedgehog signaling proteins
Article Snippet: For immunostaining in all experiments, fixed cells were blocked with 0.5% bovine serum albumin (BSA; A3912; Sigma) in PBS with 0.5% NP-40 (IGEPAL, 18896; Sigma), 1 mM MgCl 2 and 1 mM NaN 3 for 1 hour at room temperature. .. Cells were incubated overnight in primary antibodies diluted in blocking buffer: mouse anti-Smoothened (1:500; sc-166685; Santa Cruz Biotechnologies), rabbit anti-ARL13B (1:500; 177-1-AP; ProteinTech), mouse anti-ARL13B (1:300; N295B/66; Developmental Studies Hybridoma Bank, University of Iowa), goat anti-CEP192 (1:2000; gift from Andrew Holland’s lab), rabbit anti-Phospho-Smoothened (1:300; 7TM0239A-IC; 7TM Antibodies), rabbit anti-GPR161 (1:300; 29328-1-AP; ProteinTech), rabbit anti-INPP5E (1:200; 17797-1-AP; ProteinTech), rabbit anti-IFT88 (1:200; 13967-1-AP; ProteinTech), and rabbit anti-Pericentrin (1:2000; ab4448; Abcam). .. Coverslips were washed three times for 5 minutes each in PBS then incubated for 1 hour at room temperature in secondary antibodies and Hoechst-33258 (1μg/mL; Invitrogen) diluted in blocking buffer: AlexaFluor-488 donkey anti-rabbit (1:1000; A21206; Invitrogen), AlexaFlour-647 donkey anti-mouse (1:1000; A31571; Invitrogen), and AlexaFluor-594 donkey anti-goat (1:1000; A11058; Invitrogen).

Incubation:

other:

Article Title: A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology
Article Snippet: Mouse anti-Pericentrin (PCNT) , Abcam , Cat# ab28144; RRID: AB_2160664.

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